Species Mix HYE DIGEST (A+B)
Product | Catalog number |
Species Mix HYE Digest A+B | SmixDAB_1 |
Product Description
Developed by ProTec Diagnostics, the HYE Species Mix Digest (A+B) is purpose-built for the most demanding LC-MS applications. It provides a unique and reliable benchmark for the quantitative performance assessment of mass spectrometry systems.
What sets the HYE Mix apart is the targeted combination of two complementary components (Mix A and Mix B). By analyzing and directly comparing these formulations, quantitative benchmarking based on predefined protein ratios becomes possible — a level of precision that single-sample references (commonly used HeLa) cannot achieve.
Key features:
-
Quantitative comparability through defined compositions
The separate measurement of HYE Species Mix A and B allows for the precise determination of the quantification accuracy of your LC/MS system – a powerful tool for technical benchmarking and data validation. -
Broad chromatographic coverage
Balanced peptide distribution across the entire retention time window – with a strong presence in both the hydrophilic and hydrophobic regions. -
Strict quality control
Each batch is produced according to validated standards and documented by a detailed analysis certificate – ensuring consistently high quality and comparability across different batches. -
Stable and ready to use
The mix is provided in a lyophilized format, is salt-free and free of detergents.
Applications:
- System validation and performance benchmarking of LC-MS platforms
- Development and validation of LC/MS methods for quantitative analyses
- Monitoring of retention time stability and separation performance
Specifications
Final Product Type | Peptides (Protein Digest Standard of Three Species) |
For Use With | Mass Spectrometer |
Quantity 20 µg | 20 µg Mix A and 20 µg Mix B |
Quantity 100 µg | 100 µg Mix A and 100 µg Mix B |
Workflow Step | MS System Performance Method Development |
Form | Lyophilized |
Store at | -80 °C |
Usage Limitation | For Research Use Only |
Frequently asked questions (FAQs)
We recommend using the ProTec Diagnostics HYE Species Mix (Cat. SmixDAB_1) as a routine quality control sample prior to your actual sample analysis. By running several technical replicates, you can consistently monitor chromatographic and MS performance, detect run-to-run variations early, and document system stability over time.
Yes. Since the HYE Species Mix consists of three defined species (Human, Yeast, and E. coli) at known mixing ratios, it serves as an excellent external reference standard for inter-experimental and inter-laboratory normalization. Its defined stoichiometric composition makes it particularly well-suited for validating label-free quantification workflows. .