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Our Technology

The HYE Species Mix is a purpose-designed protein reference standard, developed to support reliable, reproducible, and quantitative mass spectrometry. By combining 

Human, Y​east and E. coli (HYE) peptide extracts in defined ratios (Figure 1), we created two distinct formulations to meet the diverse needs of proteomics workflows and MS performance evaluation.

• HYE-Mix A: 65% human , 30% yeast,  5% E. coli ​

    

• HYE-Mix B: 65% human , 15% yeast,  20% E. coli

HYE Species Mix A​​  HYE Species Mix ​​​​B  HYE Species M​​​​​​ix A+B​​

Ready-To Use - Designed for Your Daily MS Routine

Both mixes are easy to handle and ready-to-use, making them the ideal choice for routine application without additional preparation.

Figure 1: Two HYE Species Mix (A & B) with two different formulations containing human , yeast and E. coli peptides in species specific ratios.

Figure 2: Streamlined proteomics worklfow - simply resuspend the HYE Species Mix and it is ready for MS injection.

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LC/MS Performance: Peptide Identifcation (top) and Protein Identification (bottom)

High depth of proteome coverage and consistent quantification accuracy, from 1000 ng down to 100 pg due to the optimized peptide digest and the well-balanced species composition.

• At 1000 ng load, more than 16,000 protein groups were identified (>9,000 human, >4,500 yeast, >2,500 E. coli).

• Even at 100 pg, over 6,500 protein groups and >40,000 peptides were still identified.

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Reliable Benchmark

• Defined protein ratios are consistently reproduced in experimental data  across all tested loading amounts

• Accurate results even at very low sample amounts

• Robust MS1 quantification at ≤ 2 ng, MS2 quantification at ≥ 10 ng