Species Mix HYE DIGEST solo (A or B)

The HYE Species Mix Digest (A+B) from ProTec Diagnostics has been specifically designed for demanding LC-MS applications and offers a unique opportunity for quantitative performance assessment of mass spectrometric systems. Only through the targeted combination and direct comparison of the two components to be analyzed separately (Mix A and Mix B) is quantitative benchmarking based on predefined protein ratios made possible. This makes the product a powerful tool for method development, system comparisons, and quality assurance in proteomics.

Product features:

• Quantitative comparability through defined compositions
  The separate measurement of HYE Species Mix A and B allows for the precise determination of the quantification accuracy of your LC/MS system – a powerful tool for technical benchmarking and data validation.
• Broad chromatographic coverage
  The combined digestion provides a balanced peptide distribution across the entire retention time window – with a strong presence in both the hydrophilic and hydrophobic regions. 
• Strict Quality Control
  Each batch is produced according to validated standards and documented with a detailed analysis certificate – ensuring consistently high quality and comparability across different batches.
• Stable and ready to use
  The mix is provided in lyophilized format, is salt-free and free of detergents.

Applications:

• System validation and performance benchmarking of LC-MS platforms
• Development and validation of LC/MS methods for quantitative analyses
• Monitoring of retention time stability and separation performance

Structure of the HYE Species Mix

Our HYE-Mix consists of a carefully balanced blend of species-specific peptide extracts and contains two components to meet the varying requirements of your experiments:

HYE-Mix A: 

65% human protein, 30% yeast protein, and 5% E. coli protein.

HYE-Mix B: 

65% human protein, 15% yeast protein, and 20% E. coli protein.

Both mixtures provide an ideal foundation for a variety of mass spectrometric applications. The balanced protein distribution enables precise analyses and helps you achieve reliable results—whether in sensitivity testing, the development of MS methods, or the quality assurance of your sample preparation.

Robust quality standard:

Based on the findings of Gaither et al. (DOI: 10.1021/acs.jproteome.1c00249), the HYE species mix was tested for the stability of the lyophilized product. The tests showed a high stability of the lyophilized product over 4 weeks at room temperature.

Furthermore, the HYE Species Mix enables precise quantitative benchmarking across all tested conditions. The defined mixing ratios of the included proteins from human, yeast, and E. coli were consistently reflected in the measurement results—even with small sample sizes. The observed protein intensities closely matched the expected ratios, which impressively underscores both the robustness of analytical workflows and the accuracy of quantification down to the individual protein level.